Degradation of agricultural ecosystems and the nutritional elements imbalance in the soil are the most important reasons for decreasing yield of Pistachio in Iran. Therefore, it is needed to research on effectiveness of biological manures on the growth and yield of Pistachio. This study was conducted to evaluate the effect of triple super phosphate (at the rates of 0, 1 and 2 g kg^-1) and Barvar-2 bio-fertilizer (at the rates of 0, and 2.5 g kg^-1) on the growth of pistachio seedling and uptake of nitrogen and phosphorus. The experiment was designed in a factorial form as randomized complete blocks with three replications in Damqan city. The results indicated that Barvar-2 bio-fertilizer application increased significantly (p≤0.01) dry weight(up to 21%), the concentration of nitrogen (up to 93%) and phosphorus (up to 41%) in shoots, seedling height (up to 14%) and root length (up to 9%), in different rates of triple super phosphate application. However, seedling height, shoot phosphorus and dry weight decreased significantly in the highest rate of triple super phosphate application (2 g kg-1). In addition, the application of both treatments had not significant effect on pH values. It is concluded that severe application of P chemical fertilizers causes a decrease on effectiveness of Barvar-2 bio-fertilizer. Finally, in order to improve the growth of pistachio seedlings, the application of Barvar-2 bio-fertilizer, alone or with very low amount of P chemical fertilizer is recommended.