This research was conducted to investigate the effect of salinity stress due to increased sodium chloride on some morphological and physiological characteristics in rootstocks and interspecific hybrids of pistachio and screening of tolerant rootstocks. The factorial experiment in a completely randomized design was carried out in greenhouse conditions. The factors included four rootstocks (Badami-Riz-e-Zarand, Ghazvini, and interspecific hybrids Integerrima × Badami-Riz-e-Zarand, Integerrima × Ghazvini) and three salinity levels (0, 100 and 200 mM of NaCl) with three replicates in coco peat and perlite media. The results showed that with increasing salinity level, stem height, leaves numbers, leaf area, leaf fresh and dry weight, stem fresh and dry weight, root fresh and dry weight, leaf relative water content, leaf potassium and the ratio of potassium to sodium decreased, and ionic leakage, proline, and leaf sodium increased. The highest stem height was observed in Integerrima × Ghazvini hybrid. In addition, the highest leaves numbers, leaf area, leaf fresh and dry weight, stem fresh and dry weight, root fresh and dry weight, leaf relative water content, leaf proline content, leaf potassium and the ratio of potassium to sodium were observed in Ghazvini rootstock and the lowest was related to Integerrima × Badami-Riz-e-Zarand hybrid. The most amount of leaf ionic leakage and sodium concentration of leaves were observed in Integerrima × Badami-Riz-e-Zarand hybrid. It seems that Ghazvini and Integerrima × Ghazvini hybrid are more tolerant and Integerrima × Badami-Riz-e-Zarand hybrid is more sensitive to salinity stress.