One of the important steps in the economic production of fruit trees, especially pistachio is pollination and inoculation. Therefore, it is necessary to conduct experiments to improve the quality and quantity of the fruit. In order to investigate the effects of free and controlled pollination of pollen grains of domestic and Integerrima species on some quantitative and qualitative characteristics of Fandoghi pistachio fruit an experiment was conducted in a factorial arrangement in Completely Randomized Design with three replications in one of Astan Ghods Razavi Pistachio Gardens in Bardaskan City Executed in 2017. Experimental treatments consisted of two methods of injection pollinators and the use of a brush with four flour-pollen combinations with control with three replications. The results of statistical analysis showed that the use of free pollen of domestic species had a significant positive effect on most of the traits compared to other Integerrima pollen treatments. On the other hand, Integerrima pollen showed better results in IF4 treatment (0.50 g of Integerrima pollen + 0.25 g flour) than control and other treatments. Also, the highest reduction of traits was observed in IF1 treatment (2 g of Integerrima pollen + 0.25 g of flour) compared to other treatments in the traits of deformity. In addition, the injection pollinator method showed more favorable effects than the brush pollinator method in most traits except deformed and half kernel traits. Based on the results of the present study, it seems that using IF4 treatment in the injection pollination method can increase some traits such as the final number of fruits, kernel weight and reduce the percentage of fruit drop and indehiscent in pistachio orchards.