Almost all biotic and abiotic stresses appear in the form of nutritional stress (deficiency or toxicity) in plants. In this research, the effect of three levels of saline irrigation water (8, 12 and 16 dS/m), two pistachio cultivars, Akbari and Daneshmandi, and two native haloalkaliphilic bacterial strains at four levels (each alone, both strains and control) , was carried out on the concentration of some nutrients and their ratios in pistachio seedlings, in the factorial split-plot arrangement based on a randomized complete design in three replications (each replication includes two seedlings), in garden conditions in 2021-2022. The investigated elements included nitrogen, magnesium, sodium, chlorine, iron, manganese, copper, and the ratios of Na+/K+, Ca2+/N and Ca2+/Mg2+ in the seedlings. The concentration of most of the investigated elements and ratios increased from 8 to 12 dS/m and decreased at 16 dS/m. The highest concentration of leaf magnesium (1.28%), iron (310.98 mg/kg) and copper (14.31 mg/kg) was observed in Daneshmandi and Akbari cultivar increased nitrogen (2.60%), calcium (1.82%), manganese (13.15 mg/kg) absorption and showed a better effect on reducing the absorption of sodium and chlorine ions and the ratio of sodium to potassium.Alkalibacillus haloalkaliphilus bacterium was more successful in absorbing microelements and magnesium; However, Virgibacillus marismortui showed higher efficiency in increasing nitrogen absorption and calcium to-nitrogen ratio and decreasing sodium absorption. In total, the results showed that the concentration of leaf nutrients before being affected by salinity levels was dependent on the type of bacteria, elements chemistry, and pistachio cultivars' tolerance to salinity.